Quantitative proteome analysis of mouse liver lysosomes provides evidence for mannose 6-phosphate-independent targeting mechanisms of acid hydrolases in …
S Markmann, S Krambeck, CJ Hughes… - Molecular & Cellular …, 2017 - ASBMB
The efficient receptor-mediated targeting of soluble lysosomal proteins to lysosomes
requires the modification with mannose 6-phosphate (M6P) residues. Although the absence
of M6P results in misrouting and hypersecretion of lysosomal enzymes in many cells, normal
levels of lysosomal enzymes have been reported in liver of patients lacking the M6P-
generating phosphotransferase (PT). The identity of lysosomal proteins depending on M6P
has not yet been comprehensively analyzed. In this study we purified lysosomes from liver of …
requires the modification with mannose 6-phosphate (M6P) residues. Although the absence
of M6P results in misrouting and hypersecretion of lysosomal enzymes in many cells, normal
levels of lysosomal enzymes have been reported in liver of patients lacking the M6P-
generating phosphotransferase (PT). The identity of lysosomal proteins depending on M6P
has not yet been comprehensively analyzed. In this study we purified lysosomes from liver of …
