Clinical usefulness of a novel C1q assay to detect immunoglobulin G antibodies capable of fixing complement in sensitized pediatric heart transplant patients
C Chin, GE Chen, F Sequeria, G Berry, S Siehr… - The Journal of heart and …, 2011 - Elsevier
BACKGROUND:: Donor-specific antibodies (DSA) against human leukocyte antigens
complicate transplantation with the potential for acute antibody-mediated rejection (AMR).
Complement-fixing antibodies are required to initiate the complement cascade. Not all
DSAs, however, can fix complement. METHODS:: A novel C1q assay was developed to
detect the sub-set of immunoglobulin G (IgG) antibodies capable of fixing complement. Sera
from 18 pediatric heart transplant patients were analyzed for DSAs using a Luminex platform …
complicate transplantation with the potential for acute antibody-mediated rejection (AMR).
Complement-fixing antibodies are required to initiate the complement cascade. Not all
DSAs, however, can fix complement. METHODS:: A novel C1q assay was developed to
detect the sub-set of immunoglobulin G (IgG) antibodies capable of fixing complement. Sera
from 18 pediatric heart transplant patients were analyzed for DSAs using a Luminex platform …