A novel mitochondrial C1-tetrahydrofolate synthetase is upregulated in human colon adenocarcinoma

T Sugiura, Y Nagano, T Inoue, K Hirotani - Biochemical and biophysical …, 2004 - Elsevier
T Sugiura, Y Nagano, T Inoue, K Hirotani
Biochemical and biophysical research communications, 2004Elsevier
To seek the genes involved in the development of colorectal cancer, we analyzed the
microarray gene expression profiles of human normal and cancerous colon tissues using
the BioExpress database platform. Through the analysis we found one gene named
DKFZp586G1517 that was upregulated in colon adenocarcinomas. The full-length cDNA of
the DKFZp586G1517 cloned by polymerase chain reaction (PCR) encodes a protein with
978 amino acids, which is homologous to the human cytosolic C1-tetrahydrofolate …
To seek the genes involved in the development of colorectal cancer, we analyzed the microarray gene expression profiles of human normal and cancerous colon tissues using the BioExpress database platform. Through the analysis we found one gene named DKFZp586G1517 that was upregulated in colon adenocarcinomas. The full-length cDNA of the DKFZp586G1517 cloned by polymerase chain reaction (PCR) encodes a protein with 978 amino acids, which is homologous to the human cytosolic C1-tetrahydrofolate synthetase and contains a mitochondrial target signal at N-terminus. The gene product expressed in 293 cells was localized in mitochondria and processed at the predicted signal cleavage site, supporting the idea that DKFZp586G1517 is a novel mitochondrial C1-tetrahydrofolate synthetase (mtC1-THFS). The overexpression of mtC1-THFS in 293 cells stimulated the colony formation. These results suggest that mtC1-THFS may participate in the progression of colorectal cancer by conferring growth advantage and could be a new molecular target for cancer therapy.
Elsevier