MHC-restricted, glycopeptide-specific T cells show specificity for both carbohydrate and peptide residues

MB Deck, P Sjölin, ER Unanue… - The Journal of …, 1999 - journals.aai.org
MB Deck, P Sjölin, ER Unanue, J Kihlberg
The Journal of Immunology, 1999journals.aai.org
We examined the antigenic specificity of two T cell hybridomas elicited against the
disaccharide galabiose attached to the fifth residue of the IA k binding peptide 52–61 of
lysozyme. By making changes in the saccharide molecule and in the peptide, we conclude
that the outer galactose residue of the galabiose moiety is directly recognized by the T cells
together with the exposed side chains of the peptide. The overall spatial display of this
galactose moiety on the 52–61 peptide is likewise important.
Abstract
We examined the antigenic specificity of two T cell hybridomas elicited against the disaccharide galabiose attached to the fifth residue of the IA k binding peptide 52–61 of lysozyme. By making changes in the saccharide molecule and in the peptide, we conclude that the outer galactose residue of the galabiose moiety is directly recognized by the T cells together with the exposed side chains of the peptide. The overall spatial display of this galactose moiety on the 52–61 peptide is likewise important.
journals.aai.org