[HTML][HTML] Nck and phosphatidylinositol 4, 5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2/3 pathway
R Rohatgi, P Nollau, HYH Ho, MW Kirschner… - Journal of Biological …, 2001 - ASBMB
The Wiskott-Aldrich syndrome protein (WASP) and its relative neural WASP (N-WASP)
regulate the nucleation of actin filaments through their interaction with the Arp2/3 complex
and are regulated in turn by binding to GTP-bound Cdc42 and phosphatidylinositol 4, 5-
bisphosphate. The Nck Src homology (SH) 2/3 adaptor binds via its SH3 domains to a
proline-rich region on WASP and N-WASP and has been implicated in recruitment of these
proteins to sites of tyrosine phosphorylation. We show here that Nck SH3 domains …
regulate the nucleation of actin filaments through their interaction with the Arp2/3 complex
and are regulated in turn by binding to GTP-bound Cdc42 and phosphatidylinositol 4, 5-
bisphosphate. The Nck Src homology (SH) 2/3 adaptor binds via its SH3 domains to a
proline-rich region on WASP and N-WASP and has been implicated in recruitment of these
proteins to sites of tyrosine phosphorylation. We show here that Nck SH3 domains …