Publisher Summary This chapter focuses on the analysis of circular dichroism (CD) data to
determine thermodynamic parameters of folding, binding constants, and estimates of
secondary structure. Proteins and polypeptides have CD bands in the far ultraviolet region
that arise mainly from the amides of the protein backbone and are sensitive to their
conformations. Proteins have CD bands in the near ultraviolet and visible regions, which
arise from aromatic amino acids and prosthetic groups. CD can be used to determine the …