[CITATION][C] Regulation of the glycine cleavage system in isolated rat liver mitochondria.
RK Hampson, LL Barron, MS Olson - Journal of Biological Chemistry, 1983 - Elsevier
RK Hampson, LL Barron, MS Olson
Journal of Biological Chemistry, 1983•ElsevierMATERIALS AND METHODS Mitochondria used in these studies were isolated (27) from
livers obtained from male Sprague-Dawley rats (200-250 g) fed a standard laboratory chow
ad libitum. The liver was homogenized in a medium consisting of mannitol, 0.225 M;
sucrose, 0.075 M; and ethylene glycol bis (P-arninoethy1 ether)-N, N, N', N'-tetraacetate (0.1
mM). Subsequent washings and the final resuspension employed a similar medium
livers obtained from male Sprague-Dawley rats (200-250 g) fed a standard laboratory chow
ad libitum. The liver was homogenized in a medium consisting of mannitol, 0.225 M;
sucrose, 0.075 M; and ethylene glycol bis (P-arninoethy1 ether)-N, N, N', N'-tetraacetate (0.1
mM). Subsequent washings and the final resuspension employed a similar medium
MATERIALS AND METHODS Mitochondria used in these studies were isolated (27) from livers obtained from male Sprague-Dawley rats (200-250 g) fed a standard laboratory chow ad libitum. The liver was homogenized in a medium consisting of mannitol, 0.225 M; sucrose, 0.075 M; and ethylene glycol bis (P-arninoethy1 ether)-N, N, N’, N’-tetraacetate (0.1 mM). Subsequent washings and the final resuspension employed a similar medium
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