[HTML][HTML] Hydroxyurea arrests DNA replication by a mechanism that preserves basal dNTP pools
A Koç, LJ Wheeler, CK Mathews, GF Merrill - Journal of Biological …, 2004 - ASBMB
The relationship between dNTP levels and DNA synthesis was investigated using α factor-
synchronized yeast treated with the ribonucleotide reductase inhibitor hydroxyurea (HU).
Although HU blocked DNA synthesis and prevented the dNTP pool expansion that normally
occurs at G 1/S, it did not exhaust the levels of any of the four dNTPs, which dropped to
about 80% of G 1 levels. When dbf4 yeast that are ts for replication initiation were allowed to
preaccumulate dNTPs at 37° C before being released to 25° C in the presence of HU, they …
synchronized yeast treated with the ribonucleotide reductase inhibitor hydroxyurea (HU).
Although HU blocked DNA synthesis and prevented the dNTP pool expansion that normally
occurs at G 1/S, it did not exhaust the levels of any of the four dNTPs, which dropped to
about 80% of G 1 levels. When dbf4 yeast that are ts for replication initiation were allowed to
preaccumulate dNTPs at 37° C before being released to 25° C in the presence of HU, they …