[HTML][HTML] Nuclear extracts lacking DNA-dependent protein kinase are deficient in multiple round transcription
RL Woodard, MG Anderson, WS Dynan - Journal of Biological Chemistry, 1999 - ASBMB
We have compared levels of in vitrotranscription in nuclear extracts from DNA-dependent
protein kinase (DNA-PK)-deficient and DNA-PK-containing Chinese hamster ovary cell
lines. DNA-PK-deficient cell lines are radiosensitive mutants lacking either the catalytic
subunit or the 80-kDa subunit of the Ku protein regulatory component. Extracts from DNA-PK-
deficient cell lines had a 2–7-fold decrease in the level of in vitro transcription when
compared with matched controls. This decrease was observed with several promoters …
protein kinase (DNA-PK)-deficient and DNA-PK-containing Chinese hamster ovary cell
lines. DNA-PK-deficient cell lines are radiosensitive mutants lacking either the catalytic
subunit or the 80-kDa subunit of the Ku protein regulatory component. Extracts from DNA-PK-
deficient cell lines had a 2–7-fold decrease in the level of in vitro transcription when
compared with matched controls. This decrease was observed with several promoters …