Separation and quantification of cholesterol and major phospholipid classes in human semen by high-performance liquid chromatography and light-scattering …
G Grizard, B Sion, D Bauchart, D Boucher - Journal of Chromatography B …, 2000 - Elsevier
A high-performance liquid chromatographic method coupled with light-scattering detection
for the separate and accurate quantification of cholesterol and main phospholipid classes
was applied to human spermatozoa and seminal plasma (SP). This method is based on
normal-phase chromatography with silica gel as stationary phase and a ternary gradient
with hexane, mixtures of chloroform–methanol and water as mobile phase. Lipids are
separated with a good resolution and a high reproducibility. About 5· 106 spermatozoa or 25 …
for the separate and accurate quantification of cholesterol and main phospholipid classes
was applied to human spermatozoa and seminal plasma (SP). This method is based on
normal-phase chromatography with silica gel as stationary phase and a ternary gradient
with hexane, mixtures of chloroform–methanol and water as mobile phase. Lipids are
separated with a good resolution and a high reproducibility. About 5· 106 spermatozoa or 25 …
