Activation of the neutrophil NADPH oxidase requires translocation of cytosolic proteins p47
phox, p67 phox, and Rac to the plasma membrane or phagosomal membrane, where they
assemble with membrane-bound flavocytochrome b. During this process, it appears that p47
phox undergoes conformational changes, resulting in the exposure of binding sites involved
in assembly and activation of the oxidase. In the present study, we have directly evaluated
activation-induced conformational changes in p47 phox using tryptophan fluorescence and …