Conformational changes of the leukocyte NADPH oxidase subunit p47phox during activation studied through its intrinsic fluorescence
HS Park, JW Park - Biochimica et Biophysica Acta (BBA)-Protein Structure …, 1998 - Elsevier
The leukocyte NADPH oxidase of neutrophils is a membrane-bound enzyme that catalyzes
the production of O− 2 from oxygen using NADPH as the electron donor. Dormant in resting
neutrophils, the enzyme acquires catalytic activity when the cells are exposed to appropriate
stimuli. During activation, the cytosolic oxidase components p47phox and p67phox migrate
to the plasma membrane, where they associate with cytochrome b558, a membrane-
integrated flavohemoprotein, to assemble the active oxidase. Oxidase activation can be …
the production of O− 2 from oxygen using NADPH as the electron donor. Dormant in resting
neutrophils, the enzyme acquires catalytic activity when the cells are exposed to appropriate
stimuli. During activation, the cytosolic oxidase components p47phox and p67phox migrate
to the plasma membrane, where they associate with cytochrome b558, a membrane-
integrated flavohemoprotein, to assemble the active oxidase. Oxidase activation can be …
