Coated charcoal immunoassay of insulin
V Herbert, KS Lau, CW Gottlieb… - The Journal of Clinical …, 1965 - academic.oup.com
V Herbert, KS Lau, CW Gottlieb, SJ Bleicher
The Journal of Clinical Endocrinology & Metabolism, 1965•academic.oup.comCharcoal premixed with dextran of average molecular weight 80,000 almost instantly
adsorbs free insulin but rejects antibody-bound insulin. The use of such dextran-coated
charcoal makes simpler and more rapid the immunoassay of insulin in biologic fluids, using
radioisotope dilution with 131I-insulin and “biopsy” of the insulin pool by antibody to insulin.
The procedure here described yields a straight line graph when insulin added is plotted
against insulin recovered.
adsorbs free insulin but rejects antibody-bound insulin. The use of such dextran-coated
charcoal makes simpler and more rapid the immunoassay of insulin in biologic fluids, using
radioisotope dilution with 131I-insulin and “biopsy” of the insulin pool by antibody to insulin.
The procedure here described yields a straight line graph when insulin added is plotted
against insulin recovered.
Charcoal premixed with dextran of average molecular weight 80,000 almost instantly adsorbs free insulin but rejects antibody-bound insulin. The use of such dextran-coated charcoal makes simpler and more rapid the immunoassay of insulin in biologic fluids, using radioisotope dilution with 131I-insulin and “biopsy” of the insulin pool by antibody to insulin. The procedure here described yields a straight line graph when insulin added is plotted against insulin recovered.
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