[HTML][HTML] Role of hexosamine biosynthesis in glucose-mediated up-regulation of lipogenic enzyme mRNA levels: effects of glucose, glutamine, and glucosamine on …
JM Rumberger, T Wu, MA Hering, S Marshall - Journal of biological …, 2003 - ASBMB
Glucose uptake into adipose and liver cells is known to up-regulate mRNA levels for various
lipogenic enzymes such as fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC). To
determine whether the hexosamine biosynthesis pathway (HBP) mediates glucose
regulation of mRNA expression, we treated primary cultured adipocytes for 18 h with insulin
(25 ng/ml) and either glucose (20 mm) or glucosamine (2 mm). A ribonuclease protection
assay was used to quantitate mRNA levels for FAS, ACC, and glycerol-3-P dehydrogenase …
lipogenic enzymes such as fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC). To
determine whether the hexosamine biosynthesis pathway (HBP) mediates glucose
regulation of mRNA expression, we treated primary cultured adipocytes for 18 h with insulin
(25 ng/ml) and either glucose (20 mm) or glucosamine (2 mm). A ribonuclease protection
assay was used to quantitate mRNA levels for FAS, ACC, and glycerol-3-P dehydrogenase …
