[HTML][HTML] Phosphorylation of insulin receptor substrate-1 (IRS-1) by protein kinase B positively regulates IRS-1 function
K Paz, YF Liu, H Shorer, R Hemi, D LeRoith… - Journal of Biological …, 1999 - ASBMB
Incubation of cells with insulin leads to a transient rise in Tyr phosphorylation of insulin
receptor substrate (IRS) proteins, accompanied by elevation in their Ser (P)/Thr (P) content
and their dissociation from the insulin receptor (IR). Wortmannin, a phosphatidylinositol 3-
kinase inhibitor, selectively prevented the increase in Ser (P)/Thr (P) content of IRS-1, its
dissociation from IR, and the decrease in its Tyr (P) content following 60 min of insulin
treatment. Four conserved phosphorylation sites within the phosphotyrosine binding/SAIN …
receptor substrate (IRS) proteins, accompanied by elevation in their Ser (P)/Thr (P) content
and their dissociation from the insulin receptor (IR). Wortmannin, a phosphatidylinositol 3-
kinase inhibitor, selectively prevented the increase in Ser (P)/Thr (P) content of IRS-1, its
dissociation from IR, and the decrease in its Tyr (P) content following 60 min of insulin
treatment. Four conserved phosphorylation sites within the phosphotyrosine binding/SAIN …
