CD20 is a molecular target for scFvFc: zeta receptor redirected T cells: implications for cellular immunotherapy of CD20+ malignancy
M Jensen, G Tan, S Forman, AM Wu… - Biology of blood and …, 1998 - tctjournal.org
The CD20 molecule was evaluated as a B-cell lymphoma target epitope for T cells
expressing a CD20-specific single-chain FvFc-zeta (scFvFc: zeta) chimeric receptor. A cDNA
construct consisting of a murine kappa leader sequence, CD20-specific scFv, human
immunoglobulin (Ig) G1 hinge-C (H) 2-C (H) 3, the human CD4 transmembrane, and the
intracellular signaling domain of the human CD3 complex's zeta chain was synthesized by
polymerase chain reaction splice-overlap extension. The human CD4+ Jurkat cell line was …
expressing a CD20-specific single-chain FvFc-zeta (scFvFc: zeta) chimeric receptor. A cDNA
construct consisting of a murine kappa leader sequence, CD20-specific scFv, human
immunoglobulin (Ig) G1 hinge-C (H) 2-C (H) 3, the human CD4 transmembrane, and the
intracellular signaling domain of the human CD3 complex's zeta chain was synthesized by
polymerase chain reaction splice-overlap extension. The human CD4+ Jurkat cell line was …
