Adsorption to fibrin of native fragments of known primary structure from human plasminogen
S Thorsen, I Clemmensen, L Sottrup-Jensen… - … et Biophysica Acta (BBA …, 1981 - Elsevier
Limited proteolysis of native Glu-plasminogen with pancreatic elastase produced three
major fragments, K1+ 2+ 3, K4, K5-light chain (miniplasminogen). Fibrin-binding was
determined by clotting fibrinogen in the presence of 125 I-labelled fragments and measuring
125 I in the washed fibrin and in the supernatant. Of the fragments miniplasminogen showed
the highest fibrin-binding, the strength of which was intermediate between those of Glu-
plasminogen and Lys-plasminogen. The fibrin-binding of all three fragments was decreased …
major fragments, K1+ 2+ 3, K4, K5-light chain (miniplasminogen). Fibrin-binding was
determined by clotting fibrinogen in the presence of 125 I-labelled fragments and measuring
125 I in the washed fibrin and in the supernatant. Of the fragments miniplasminogen showed
the highest fibrin-binding, the strength of which was intermediate between those of Glu-
plasminogen and Lys-plasminogen. The fibrin-binding of all three fragments was decreased …
