A novel assay for 5'-nucleotidase using 1, N6-etheno-AMP as substrate, and comments on the properties of the reaction product, ethenoadenosine.
Z Jamal, A Afkham-Ebrahimi, ED Saggerson - Biochemical Journal, 1988 - ncbi.nlm.nih.gov
Z Jamal, A Afkham-Ebrahimi, ED Saggerson
Biochemical Journal, 1988•ncbi.nlm.nih.govA novel assay for 5'-nucleotidase is described in which 1, N6-etheno-AMP is converted into
ethenoadenosine. The product ethenoadenosine is neither a substrate for nor an inhibitor of
adenosine deaminase. Ethenoadenosine appears to have little effect at adenosine
receptors on adipose-tissue cells.
ethenoadenosine. The product ethenoadenosine is neither a substrate for nor an inhibitor of
adenosine deaminase. Ethenoadenosine appears to have little effect at adenosine
receptors on adipose-tissue cells.
Abstract
A novel assay for 5'-nucleotidase is described in which 1, N6-etheno-AMP is converted into ethenoadenosine. The product ethenoadenosine is neither a substrate for nor an inhibitor of adenosine deaminase. Ethenoadenosine appears to have little effect at adenosine receptors on adipose-tissue cells.
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