[HTML][HTML] Phosphatidylinositol 3-kinase signaling inhibits DAF-16 DNA binding and function via 14-3-3-dependent and 14-3-3-independent pathways
CM Cahill, G Tzivion, N Nasrin, S Ogg, J Dore… - Journal of Biological …, 2001 - ASBMB
In Caenorhabditis elegans, an insulin-like signaling pathway to phosphatidylinositol 3-
kinase (PI 3-kinase) and AKT negatively regulates the activity of DAF-16, a Forkhead
transcription factor. We show that in mammalian cells, C. elegans DAF-16 is a direct target of
AKT and that AKT phosphorylation generates 14-3-3 binding sites and regulates the
nuclear/cytoplasmic distribution of DAF-16 as previously shown for its mammalian homologs
FKHR and FKHRL1. In vitro, interaction of AKT-phosphorylated DAF-16 with 14-3-3 prevents …
kinase (PI 3-kinase) and AKT negatively regulates the activity of DAF-16, a Forkhead
transcription factor. We show that in mammalian cells, C. elegans DAF-16 is a direct target of
AKT and that AKT phosphorylation generates 14-3-3 binding sites and regulates the
nuclear/cytoplasmic distribution of DAF-16 as previously shown for its mammalian homologs
FKHR and FKHRL1. In vitro, interaction of AKT-phosphorylated DAF-16 with 14-3-3 prevents …
